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CABRI:DSMZ_MUTZ |
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Accession_number |
ACC 296
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Cell_line_name |
A4-1025
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Brief_description |
confirmed as mouse with IEF of AST, NP described as secreting a mouse
monoclonal antibody (IgG 2a ) detecting all fibers in skeletal muscle and myosin heavy
chains on Western blots and detecting mammalian, chicken, zebrafish, axolotl,
xenopus skeletal muscle myosin heavy chains; the cell line was established in 1985 by
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Description |
mouse hybridoma (anti-fiber/myosin) described as secreting a mouse monoclonal
antibody (IgG 2a ) detecting all fibers in skeletal muscle and myosin heavy chains on
Western blots and detecting mammalian, chicken, zebrafish, axolotl, xenopus skeletal
muscle myosin heavy chains; the cell line was established in 1985 by fusion of
X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized
with human skeletal muscle myosin confirmed as mouse with IEF of AST, NP
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Depositor |
Dr. S. M. Hughes, King's College, London, England
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Morphology |
round single cells in suspension, partly in clusters, lightly adherent
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Culture_conditions |
90% Dulbecco's MEM (4.5 g/L glucose) + 10% FBS + 2 mM L-glutamine + 1 mM
sodium pyruvate + 1x non-essential amino acids split saturated culture 1:5 to 1:10
every 2-3 days; seed out at about 1.0-1.5 x 105 cells/ml at 37C with 5% CO2 cell
harvest of about 0.5-1.0 x 106 cells/ml
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Viruses |
ELISA: reverse transcriptase positive
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Properties |
secretion of a IgG 2a monoclonal antibody
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Release_conditions |
-
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Hazard |
-
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Storage |
frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 106 cells/ampoule
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Doubling_time |
about 40-50 hours
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Mycoplasma |
negative in DAPI, microbiological culture, RNA hybridization assays
Cytogenetics murine hypertetraploid karyotype with 5% polyploidy - 84(79-84) - one large
submetacentric and up to 5 centric fusion markers present
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Bibliographic_reference |
Dan-Goor et al., J Muscle Res Cell Motil, 11: 61 [PMID: 1698201
] |
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